Unlockable characters(Survival mode):
Unlock the following characters in Survival mode by defeating them in Vs. mode at the specified locations:
CHARACTER – HOW TO UNLOCK
Bazooka Marine – Defeat the Bazooka Marine in the Fossil Beach.
Blue Demon General – Defeat the Blue Demon General in the Cave Zone.
Cluster Demon Soldier – Defeat the Cluster Demon Soldier in the Cave Zone.
Crimson Demon General – Defeat the Crimson Demon General in the Cave Zone.
Knuckle Pirate – Defeat the Knuckle Pirate in the Autumn Colored Ruins.
Marine Corps Chief – Defeat the Marine Corps Chief in the Mystery Jungle.
Normal Marine – Defeat the Normal Marine in the Fossil Beach.
Pistol Pirate – Defeat the Pistol Pirate in the Mystery Jungle.
Saber Pirate – Defeat the Saber Pirate in the Mystery Jungle.
Sword Demon Soldier – Defeat the Sword Demon Soldier in the Cave Zone.
World Government Agent – Defeat the World Government Agent in the Autumn Colored Ruins.
Unlockable characters(Vs. mode):
Unlock the following characters in Vs. mode by performing the corresponding tasks:
CHARACTER – HOW TO UNLOCK
Aokiji – Defeat Aokiji in Land To Heaven.
Arlong – Defeat Arlong in the Cave area in Story mode.
Bon Clay – Unlock the Orb Spot at Fossil Beach that leads to the Red Orb spot, then defeat him.
Calgara – Unlock the Orb Spot at the Mystery Jungle that leads to the Red Orb spot, then defeat him.
Crocodile – Defeat Crocodile in the Cave area in Story mode.
Eneru – Defeat Eneru at the Autumn Colored Ruins in Story mode.
Evil Guardian – Defeat the final Boss in Beginning and End.
Evil Master Beast – Defeat the final Boss in Beginning and End.
Kaku – Defeat Kaku in the Jungle area.
Lucci – Defeat Lucci in Land To Heaven.
Mihawk – Defeat Mihawk in the Fossil Beach area in Story mode.
Paulie – Unlock the Orb Spot at the Cave of Darkness that leads to the Red Orb spot, then defeat him.
Portgas D. Ace – Defeat Portgas D. Ace in the Silver Mountains.
Rampaging Chopper – Unlock the Orb Spot at Silver Mountains that leads to the optional Red Orb spot, then defeat him.
Rob Lucci – Defeat Rob Lucci in Land To Heaven.
Shanks – Defeat Shanks in the Silver Mountains.
Smoker – Defeat Smoker just before entering Fossil Beach in Story mode.
Spandam – Defeat Spandam in the Autumn Colored Ruins in Story mode.
Vivi – Unlock the Orb Spot at the Autumn Colored Ruins that leads to the Red Orb spot, then defeat her.
Whitebeard – Unlock the Orb Spot in the Land to Heaven that leads to the Red Orb spot, then defeat him.